Isolation in Cell Culture
نویسندگان
چکیده
The aetiology of inflammatory bowel disease is at present obscure, but there is evidence that transmissible agents such as bacteria or viruses may be associated with this condition.' 4 Chlamydia trachomatis agents are known to infect the rectum5 6 and lymphogranuloma venereum (LGV) serotypes can cause chronic inflammation with fistula formation similar to manifestations of Crohn's disease. Previous studies reporting the presence of circulating antibodies to various chlamydial serotypes in patients with Crohn's disease have yielded conflicting results.7-9 In this study we have investigated the possible association of C. trachomatis with inflammatory bowel disease by attempting to isolate chlamydiae in cell culture, by searching for chlamydial antigen in diseased tissue using an immunohistological technique, and by detecting antichlamydial antibody using a serological technique, in patients and healthy controls.
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